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anti igg3 goat anti mouse abs  (Bethyl)
93
Bethyl anti igg3 goat anti mouse abs
FIGURE 7. Enhanced in vivo immune responses in DUSP14-deficient (DUSP14-KO) mice. WT and DUSP14-KO mice were immunized with KLH emulsified in alum. Seven days later, enlarged lymph nodes were isolated and restimulated with KLH for 72 h. (A) Cell proliferation was measured using [3H]thymidine incorporation. (B) IL-2, IL-4, and IFN-g levels in supernatants were measured using ELISA. Three mice were an- alyzed for each genotype. Data are mean 6 SEM. (C) WT and DUSP14- KO mice were immunized as before. Sera were collected at 14 d after the primary immunization. NP-specific IgM, IgG1, IgG2a, IgG2b, and <t>IgG3</t> Abs were determined by ELISA. Results are presented relative to those of normal serum from a WT mouse. Five mice were analyzed for each ge- notype. Data are mean 6 SEM. *p , 0.05, two-tailed t test.
Anti Igg3 Goat Anti Mouse Abs, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 7. Enhanced in vivo immune responses in DUSP14-deficient (DUSP14-KO) mice. WT and DUSP14-KO mice were immunized with KLH emulsified in alum. Seven days later, enlarged lymph nodes were isolated and restimulated with KLH for 72 h. (A) Cell proliferation was measured using [3H]thymidine incorporation. (B) IL-2, IL-4, and IFN-g levels in supernatants were measured using ELISA. Three mice were an- alyzed for each genotype. Data are mean 6 SEM. (C) WT and DUSP14- KO mice were immunized as before. Sera were collected at 14 d after the primary immunization. NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 Abs were determined by ELISA. Results are presented relative to those of normal serum from a WT mouse. Five mice were analyzed for each ge- notype. Data are mean 6 SEM. *p , 0.05, two-tailed t test.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation.

doi: 10.4049/jimmunol.1300989

Figure Lengend Snippet: FIGURE 7. Enhanced in vivo immune responses in DUSP14-deficient (DUSP14-KO) mice. WT and DUSP14-KO mice were immunized with KLH emulsified in alum. Seven days later, enlarged lymph nodes were isolated and restimulated with KLH for 72 h. (A) Cell proliferation was measured using [3H]thymidine incorporation. (B) IL-2, IL-4, and IFN-g levels in supernatants were measured using ELISA. Three mice were an- alyzed for each genotype. Data are mean 6 SEM. (C) WT and DUSP14- KO mice were immunized as before. Sera were collected at 14 d after the primary immunization. NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 Abs were determined by ELISA. Results are presented relative to those of normal serum from a WT mouse. Five mice were analyzed for each ge- notype. Data are mean 6 SEM. *p , 0.05, two-tailed t test.

Article Snippet: The titers of anti–NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 in the sera were measured by ELISAs (eBioscience) using NP-BSA (Biosearch Technologies) as the coating Ag, followed by incubation with anti-IgM, anti-IgG1, anti-IgG2a, anti-IgG2b, and anti-IgG3 goat anti-mouse Abs (all from Bethyl Laboratories) (19).

Techniques: In Vivo, Isolation, Enzyme-linked Immunosorbent Assay, Two Tailed Test